Volume 1, Number 2 (6-2015)                   jicr 2015, 1(2): 58-61 | Back to browse issues page


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Mohammadzade H, Khansari nejad B, Sadeghi A, Abtahi H. Cloning of Pituitary Gland Hormone in pET32a Expression Vector. jicr. 2015; 1 (2) :58-61
URL: http://jicr.arakmu.ac.ir/article-1-56-en.html

Abstract:   (859 Views)

Introduction: The aim of this study was to determine the ability of the cloning of human genes in bacterial hosts using codon optimization program and pET32a vector. Production of recombinant proteins, particularly human proteins in bacterial hosts has a great importance in genetic engineering. The advantages of this process is fast, further and cheaper production of proteins. Bacterial hosts are readily available and use of them is easy.

Methods: One of the hormones of pituitary gland as a human gene was cloned in E. coli host with thepET32a vector and finally cloned gene was confirmed by sequencing.

Results: The cloned gene matched with optimized and designed gene according to sequence and size.

Conclusion: Recombinant production of human proteins after codon optimization and by using suitable vectors such as pET32a is possible in bacterial hosts. These recombinant proteins have great similarity to natural forms and can be appropriate alternatives for using in diagnostic procedures or even pharmaceutical purposes. 

Full-Text [PDF 843 kb]   (558 Downloads)    
Type of Study: Research | Subject: Special
Received: 2015/10/25 | Accepted: 2015/10/25 | Published: 2015/10/25

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