year 2, Issue 1 (8-2016)                   jicr 2016, 2(1): 174-180 | Back to browse issues page

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Shahrokhi F S, Baazm M, Goodarzi M T, Eftekhar E, Jalali Mashayekhi F. The Effect of Resveratrol on mRNA levels of DNA polymerase beta and oxidative DNA damage in H2O2-induced human colon cancer HT-29 cells. jicr. 2016; 2 (1) :174-180
Abstract:   (10310 Views)

Introduction: Resveratrol (3,4,5-trihydroxystilbene), a polyphenol found in high levels in grape skin, has recently attracted huge attention because of its anti-carcinogenic properties. Protective effects of resveratrol against oxidative damage in DNA may be due to its ability to stimulate DNA repair pathways such as the base excision repair (BER).

Methods: This study aimed to investigate the effect of resveratrol on gene expression of DNA polymerase beta (DNA pol β), the primary polymerase involved in BER, in H2O2-inducedoxidative human colon cancer HT-29 cells. The 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-OHdG) level and mRNA expression level of DNA polymerase beta (pol β) was measured after human colon cancer HT-29 cells were

treated with 100 μM H2O2for 30 min followed by

exposure with 75 µM of resveratrol for 48 h.

Results: The level of 8-OHdG was significantly increased by H2O2 treatment, but resveratrol pretreatment of cells prior to H2O2 treatment led to a significant reduction of 8-OHdG to the levels similar to those observed in controls. Analysis of qRT -PCR data by one way ANOVA revealed that resveratrol pretreatment also caused a measurable increase in the mRNA expression of DNA polymerase beta (DNA pol β) comparing to that of H2O2-treated and control cells.

Conclusion: The cancer-preventive effects of resveratrol may be due in part to stimulation of base excision repair processes. Our data confirm that resveratrol exerts its anti-oxidant and scavenging properties through a reduction in 8-OHdG level.

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Type of Study: Research | Subject: Special
Received: 2016/06/29 | Accepted: 2016/08/31 | Published: 2016/08/31

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