year 1, Issue 2 (6-2015)                   jicr 2015, 1(2): 49-53 | Back to browse issues page

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Darabi M R, Baazm M, Babaei S, Nezhadi A. Comparison of Ethanol and Ionomycine for cleavage and development of blocked mouse two-cell embryo. jicr. 2015; 1 (2) :49-53
Abstract:   (892 Views)

Introduction: One of the most important reasons of infertility between couples is arresting of embryos in certain stage of development like two-cell stage embryos. There are different methods for activating oocytes and embryos routinely used in fertility and infertility centers. In this study, the effects of ethanol and calcium ionophore on activating of arrested two-cell stage embryos were compared.

Methods: After superovulation, female and male mice were coupled and the positive vaginal plaque mice were killed. Their uterine tubes were washed in order to gather their two-cell embryos in M16 medium. Subsequently the two-cell embryos were divided randomly to four groups. The 1st (1st control) group were incubated without any exposure, and the other embryos were exposed to 4 °C for 24 h in order to arrest in two-cell stage. The 2nd group were incubated after low temperature exposure. The 3rd group were exposed to 0.1%ethanol for 5 minutes and the 4th group to 10 µM calcium ionophore for 5 minutes and subsequently were incubated.

Results: The mean percent of degenerated embryos was increased significantly with low temperature exposure between groups (P=0.005). Similarly the cleavage rate was significant between groups except between groups 3 and 4 (P=0.217). In addition, the developmental rate was significant between groups. The mean percent of hatched blastocyst were higher in group 3 compared to other groups but their differences were not significant (P=0.512).

Conclusion: Due to ethanol and calcium ionophore exposure of arrested two-cell embryos the rate of cleavage and blastocyst formation rate were increased probably because of calcium ossilation produced by chemical activators. Comparatively ethanol is more effectively on cleavage and development than calcium ionophore.

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Type of Study: Research | Subject: Special
Received: 2015/10/25 | Accepted: 2015/10/25 | Published: 2015/10/25

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